HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar
https://sielc.com/Application-HPLC-Analysis-of-Polysorbate-in-Mixture-With-Amino-Acids-and-SugarChromatogram
Description
Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.
Method Parameters
| Mobile Phase | IPA |
|---|---|
| Buffer | Formic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Glycine, Histidine, Sucrose, Polysorbate 20 |
HPLC Column Used
Primesep D, 3.2×50 mm, 5 µm, 100A