HPLC Analysis of Polar Basic and Acetic Compounds on Primesep AB Column
https://sielc.com/Application-HPLC-Analysis-of-Polar-Basic-and-Acetic-Compounds-on-Primesep-AB-ColumnChromatogram
Description
Mixture of polar acidic and basic compounds is separated on a Primesep AB mixed-mode HPLC column. Dopamine and tyrosine are retained by combination of reversed-phase and cation-exchange mechanisms. Maleic acid is retained by anion-exchange mechanism, and benzoic acid is retained by reversed-phase mechanism. Primesep AB is a trimodal column with a C12 hydrophobic chain and cation-exchange and anion exchange groups on the surface. Method utilizes UV detection but can be used with other detection techniques (ELSD, LC/MS, Corona).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Tyrosine, Dopamine, Maleic Acid, Benzoic Acid |
HPLC Column Used
Primesep AB, 4.6×150 mm, 5 µm, 100A