HPLC Analysis of Metformin
https://sielc.com/Application-HPLC-Analysis-of-MetforminChromatogram
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Description
Metformin is a biguanide-based anti-diabetic drug. It is a very polar organic compound that has no retention on reversed-phase column unless an ion-pairing reagent is employed. Metformin was retained on a Primesep 200 column based on cation-exchange mechanism. Method can be used for analysis of metformin and related substances in pharmaceutical formulations. Due to low UV activity a more UV-transparent mobile phase is required. Metformin can be also monitored by ELSD and LC/MS with corresponding mobile phase (TFA, ammonium formate or ammonium acetate).
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds | Drug, Anti-diabetic medication, Hydrophilic, Ionizable |
| Analyzing Compounds | Metformin |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A