HPLC Analysis of Histones
https://sielc.com/Application-HPLC-Analysis-of-HistonesChromatogram
Description
Histones are highly alkaline proteins. A mixture of histones was separated on Promix MP HPLC column. Basic nature of stationary phase guarantees perfect shape of histones due to shielding effect of stationary phase. No silanol interaction was observed. Method can be used for analysis and isolation of histones.
Method Parameters
| Mobile Phase | Gradient MeCN 10 – 40%, 40 min , 5 min hold |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds | Proteins, Hydrophobic, Ionizable |
| Analyzing Compounds | Histones |
HPLC Column Used
Promix MP, 4.6×250 mm, 5 µm, 100A