HPLC Analysis of Fentin on Obelisc and Primesep Mixed-Mode Columns
https://sielc.com/Application-HPLC-Analysis-of-Fentin-on-Obelisc-and-Primesep-Mixed-Mode-ColumnsChromatogram
Description
Fentin is a fungicide made up of three aromatic groups on a tin atom. A single residue method was developed using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, to be used by EURL (European Union Reference Laboratory). Fentin was analyzed on a 3 micron Primesep 100 and Obelisc R column. Primesep 100 retains using embedded acidic ion-paring groups, while Obelisc R retains using multiple ionic-pairing groups and a long hydrophobic chain. Method is LC/MS compatible and same conditions can be used to separate many different pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | F |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A