HPLC Analysis of Diphenamid on Primesep 100 and Obelisc R
https://sielc.com/Application-HPLC-Analysis-of-Diphenamid-on-Primesep-100-and-Obelisc-RChromatogram
Description
Diphenamid is an herbicide which selectively controls weedy grasses and broadleaf plants in the production of tobacco, tomatoes, peppers, cotton, and many more commercial crops. Diphenamid is a preemergence herbicide that is surface applied to soils and can be expected to control weeds for 6-8 months. Diphenamid was analyzed on two different stationary phases. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70% |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Herbicide, Insecticide, Hydrophobic, Ionizable |
| Analyzing Compounds | Diphenamid |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A