HPLC Analysis of Clopyralid
https://sielc.com/Application-HPLC-Analysis-of-ClopyralidChromatogram
Description
Clopyralid (3,6-dichloro-2-pyridinecarboxylic acid) is a selective herbicide effective against broad-leaf plants such as clovers and thistle. A single residue method for the EURL (European Union Reference Laboratory) was developed for clopyralid and other acidic compounds using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology. Retention of clopyralid was controlled on both Obelisc R and a 3 micron Primesep 100 column of the same dimension. This method is LC/MS compatible and useful for separating many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Clopyralid |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A