HPLC Analysis of Citrulline and Arginine on Primesep Columns
https://sielc.com/Application-HPLC-Analysis-of-Citrulline-and-Arginine-on-Primesep-ColumnsChromatogram
Description
Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine, Chloride |
HPLC Column Used
Primesep, 2.1×50 mm, 5 µm, 100A