HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography
https://sielc.com/Application-HPLC-Analysis-of-Cheese-Extract-Using-Mixed-Mode-ChromatographyChromatogram
Description
Primesep 100 separates the components of a Swiss cheese extract by HPLC using cation exchange and reversed phase as retention mechanisms. The amino acids, glutamic acid and proline, as well as glycine betaine were resolved in less than 10 minutes. A mobile phase gradient of water, acetonitrile (MeCN, ACN,) and phosphoric acid (H3PO4) and ultraviolet (UV) detection was used.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H3PO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Glutamic acid, Proline, Glycine betaine |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A