HPLC Analysis of Bentazon on Primesep and Obelisc Mixed-Mode Columns
https://sielc.com/Application-HPLC-Analysis-of-Bentazon-on-Primesep-and-Obelisc-Mixed-Mode-ColumnsChromatogram
Description
Bentazon is a thiadiazine herbicide which is used to control the growth of specific plants. Some plants are not affected such as alfalfa, corn and peanuts. The EURL (European Union Reference Laboratory) included bentazon in an analysis of acidic pesticides using QuEChERS method. Primesep 100 and Obelisc R were used to retain and separate bentazon from impurities. Method is LC/MS compatible and developed to be used for many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Bentazon |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A