HPLC Analysis of Azide Ion on Obelisc N Column
https://sielc.com/Application-HPLC-Analysis-of-Azide-Ion-on-Obelisc-N-ColumnChromatogram
Description
Sodium azide is used in older car airbag systems and airplane escape slides. It is an acutely toxic substance. Obelisc N mixed-mode column can retain the azide ion using MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate as buffer. Can also be UV detected at 250nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 85/15% |
|---|---|
| Buffer | AmAc pH 5- 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Anion, Hydrophilic, Ionizable |
| Analyzing Compounds | Azide |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A