HPLC Analysis of Ascorbic Acid, Retinol (Vitamin A) and Vitamin E acetate in ACE Serum Active Ingredients
https://sielc.com/Application-HPLC-Analysis-of-ACE-Serum-Active-IngredientsChromatogram
Description
Mixed-mode HPLC columns allow for separation of compounds with drastically different properties. In this application, highly hydrophilic ascorbic acid is retained and separated from highly hydrophobic retinol (Vitamin A) and Vitamin E Acetate. Ascorbic acid is retained by anion-exchange mechanism and hydrophobic compounds are retained by reversed-phase mechanism. Compounds are monitored by UV detection. Mobile phase is compatible with LC/MS and method can be used to monitor basic and acidic compounds in bio-fluids (serum, blood, urine, saliva, etc.) Retinol (Vitamin A) and Vitamin E Acetate
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 270, 325 |
| Class of Compounds | Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Ascorbic acid (Vitamin C), Retinol (Vitamin A) and Vitamin E Acetate |
HPLC Column Used
Primesep SB , 3.0×50 mm, 5 µm, 100A