HPLC Analysis of 2,4,5-T on Mixed-Mode Columns
https://sielc.com/Application-HPLC-Analysis-of-2-4-5-T-on-Mixed-Mode-ColumnsChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
2,4,5-T is a synthetic herbicide that defoliates broad-leaf plants. It was a major component of Agent Orange, a defoliant used during the Vietnam War. 2,4,5-T has been replaced by dicamba and triclopyr due to its possible toxicity. 2,4,5-T was included in a proficiency test ran by the EURL (European Union Reference Laboratory) using QuEChERS methodology on acidic pesticides. Obelisc R retained 2,4,5-T because it has a long hydrophobic chain and ionic pairing groups. Primesep 100 retains by reverse-phase and with acid ion-pairing groups. Method is LC/MS compatible and same conditions can be used on many different pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | 2,4,5-T |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A