HPLC Analysis of 17a-Ethynylestradiol
https://sielc.com/Application-HPLC-Analysis-of-17a-EthynylestradiolChromatogram
Description
17a-Ethynylestradiol or Ethynyl Estradiol (EE2) is a partially synthetic steroidal estrogen and one of the most commonly used drugs for use in oral contraceptives. EE2 is an estrogen since it activates the estrogen receptor. While dosing of EE2 has been lowered in oral contraceptives, worries about water contamination made an analysis of EE2 necessary. Obelisc R and Primesep 100 were used to demonstrate their unique modes of separation. Method is LC/MS compatible and able to be replicated for many different pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Hormone, Hydrophobic, Ionizable |
| Analyzing Compounds | 17a-Ethynylestradiol |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A