HPLC Separation of the Nonionic Surfactant Triton-X-100
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Description
Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. SHARC 1 HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved in anhydrous conditions by hydrogen bonding using acetonitrile (ACN) and methanol (MeOH) as the mobile phase with formic acid and ammonium formate (AmFm) as buffer. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (UV, ELSD, LC/MS, RI, CAD, etc.)
Method Parameters
| Mobile Phase | Gradient MeCN/MeOH – 100/0- 50/50%, 12 min |
|---|---|
| Buffer | Formic Acid 0.1% , AmFm – 0.01% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Nonionic Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Triton-X-100 |
HPLC Column Used
Sharc 1, 4.6×150 mm, 5 µm, 100A