HPLC Separation of Triclopyr
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Description
Triclopyr (3,5,6-Trichloro-2-pyridinyloxyacetic acid) is sold under the brand names Turflon, Weed-B-Gon, and Brush-B-Gon. Triclopyr controls broad leaf plants and is very similar to the pesticide it replaced 2,4,5-T. The EURL (European Union Reference Laboratory) included triclopyr in an analysis of acidic pesticides using QuEChERS method. Primesep 100 and Obelisc R were used to retain and separate triclopyr from impurities. Method is LC/MS compatible and developed to be used for many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Triclopyr |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A