HPLC Separation of Phosphorous and Phosphoric Acids
https://sielc.com/HPLC%20Separation%20of%20Phosphorous%20and%20Phosphoric%20AcidsChromatogram
Description
Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach Application Columns: Primesep D, Obelisc N Application compounds: Phosphorous Acid, Phosphoric Acid Detection technique: UV, LC/MS, ELSD/CAD
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Fungicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Phosphorous Acid, Phosphoric Acids |
HPLC Column Used
Obelisc N, 4.6×50 mm, 5 µm, 100A