HPLC Separation of Organics Acids
https://sielc.com/HPLC%20Separation%20of%20Organics%20AcidsChromatogram
Description
Primesep D separates organic acids such as fumaric, benzoic, phthalic, naphthoic, and maleic acids by a mixture of anion exchange and reversed phase. Retention times and elution order can be changed by adjusting the percentage of acetonitrile in the mobile. This can not be done by traditional ion-exchange and ion-exclusion chromatography. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Fumaric Acid, Benzoic Acid, Phthalic Acid, Maleic Acid, Naphtoic Acid |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A