HPLC Separation of Inosine and Deoxyinosine

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Chromatogram

Method Parameters

Mobile PhaseMeCN/MeOH – 97/3%
BufferFormic Acid 0.1% , AmFm – 0.01%
Flow Rate1.0 ml/min
DetectionUV, 270 nm
Class of CompoundsNucleoside monophosphate, Hydrophilic, Ionizable
Analyzing CompoundsInosine, Deoxyinosine

HPLC Column Used

Sharc 1, 3.2×100 mm, 5 µm, 100A
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