HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
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Description
Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.
Application Columns: Primesep 100 · Application compounds: Dopac, Homovanillic Acid, Dopamine · Detection technique: UV, LC/MS, ELSD/CAD
Method Parameters
| Mobile Phase | MeCN/H2O – 5/95% |
|---|---|
| Buffer | AmFm pH 3.0- 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | DOPAC, Homovanillic Acid, Dopamine |
HPLC Column Used
Primesep 100, 4.6×50 mm, 5 µm, 100A