HILIC Separation of Surfactant Triton X-100 on Obelisc N Column
https://sielc.com/Application-HILIC-Separation-of-Surfactant-Triton-X-100%20on%20Obelisc%20N%20ColumnChromatogram
Description
Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250, 230nm |
| Class of Compounds | Surfactant, Hydrophobic, Ionizable |
| Analyzing Compounds | Triton X-100 |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A