HPLC Separation of Ritonavir and Ribavirin
https://sielc.com/Application-HILIC-Separation-of-Ritonavir-and-RibavirinChromatogram
Description
· Separation type: Liquid Chromatography HILIC
Ritonavir and Ribavarin are two drugs which are very different in their properties for HPLC separation. Very hydrophobic and very hydrophilic drugs are separated on Primesep N using Hydrophilic Interaction Chromatography (HILIC). Ritonavir is an antiretroviral drug from the protease inhibitor class used to treat HIV infection and AIDS. Ritonavir is very hydrophobic and non-ionizable and retains very long in reverse phase chromatography. Ribavirin is an anti-viral drug which is active against a number of DNA and RNA viruses. It is a member of the nucleoside antimetabolite drug, which makes it very hydrophilic.
Method Parameters
| Mobile Phase | MeCN/H2O – 97/3% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 nm, MS- compatible mobile phase |
| Class of Compounds | Drug |
| Analyzing Compounds | Ritonavir, Ribavirin |
HPLC Column Used
Primesep N, 4.6×150 mm, 5 µm, 100A