HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA
https://sielc.com/Application-HILIC-Separation-of-Common-Preservatives-Citric-Acid-Ascorbic-Acid-and-EDTAChromatogram
Description
Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Citric acid, ascorbic acid and EDTA |
HPLC Column Used
Obelisc N , 4.6×150 mm, 5 µm, 100A