HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns
https://sielc.com/Application-HILIC-Retention-of-1-5-Naphthalenedisulfonic-Acid-on-Obelisc-N-Mixed-Mode-HILIC-ColumnsChromatogram
Description
Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 1,5-Naphthalenedisulfonic Acid |
HPLC Column Used
Obelisc N , 4.6×150 mm, 5 µm, 100A