Folpet Analysis on Primesep 100 and Obelisc R HPLC Columns
https://sielc.com/Application-Folpet-Analysis-on-Primesep-100-and-Obelisc-R-HPLC-ColumnsChromatogram
Description
Folpet is a fungicide which is used to prevent a variety of fungal infections such as apple scab, cherry leaf spot, rose black spot, and rose mildew. Folpet was one of many pesticides studied by the EURL-SRM, a proficiency test for single residue methods developed by the European Union Reference Laboratory. Folpet was retained and separated from impurities with Obelisc R and Primesep 100 columns. Primesep 100 contains embedded acidic ion-pairing groups and Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method can be used for dozens of other pesticides and is LC/MS compatible.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Folpet |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A