Effect of Both pH and Organic Content on a Separation of Sugars, Amino Acids, and Carboxylic Acids
https://sielc.com/Application-Effect-of-Both-PH-and-Organic-Content-on-A-Separation-of-Sugars-Amino-Acids-and-Carboxylic-AcidsChromatogram
Description
In mixed-mode HILIC chromatography, selectivity of separation can be adjusted by amount of acetonitrile, amount of buffer and buffer pH. Buffer concentration and pH will affect retention of ionizable compounds to a different degree. Retention of neutral compounds can be adjusted by the amount of acetonitrile. Carboxylic acid, three amino acids and two sugars are separated by combination of HILIC and ion-exchange mechanisms. Compounds can be monitored by ELSD, Corona (CAD), LC/MS or low UV. UV-transparent mobile phase /buffer is required for UV monitoring of this mixed-mode separation. This HPLC method can be adopted as general approach for analysis of sugars, amino acids and carboxylic acids.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Succinic Acid, Phenylalanine, Sucrose, Glycine, Aspartic Acid, Raffinose |
HPLC Column Used
Obelisc N, 4.6×150 mm, 5 µm, 100A