Effect of Acid on Separation of Xanthines
https://sielc.com/Application-Effect-of-Acid-on-Separation-of-XanthinesChromatogram
Description
Primesep B separates xanthines such as theobromine, dimethylxanthine, theophylline by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column allow retention of hydrophilic xanthines, and the reversed-phase properties retain hydrophobic compounds. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA), perchloric acid (HClO4), or sulfuric acid (H2SO4) and UV detection.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | TFA , HClO4, H2SO4 |
| Flow Rate | 1.0 ml/min |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Theobromine, 1,7 – Dimethylxanthine, Theophyline |
HPLC Column Used
Primesep B