Diclobutrazol Separation on Primesep and Obelisc HPLC Columns
https://sielc.com/Application-Diclobutrazol-Separation-on-Primesep-and-Obelisc-HPLC-ColumnsChromatogram
Description
Diclobutrazol is a fungicide based on 1,2,4-triazol. It is used in production of coffee and various cereal grains. Primesep 100 Obelisc R were used to separate diclobutrazol. Primesep 100 is a mixed-mode column which uses embedded ion-pairing groups and Obelisc can fine tune separations using both a long hydrophobic chain and ionic groups. Method is LC/MS compatible and can be used for many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Diclobutrazol |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A