Dichlorprop (2,4-DP) Analysis on Primesep and Obelisc HPLC Columns
https://sielc.com/Application-Dichlorprop-%282-4-DP%29-Analysis-on-Primesep-and-Obelisc-HPLC-ColumnsChromatogram
Description
Dichlorprop (2,4-DP) is an herbicide similar to 2,4-D. Dichlorprop is used to control weeds and can be found in many weed killers. Only the R-isomer of 2,4-dichloropropanoic acid is used as an herbicide. The EURL (European Union Reference Laboratory) included dichlorprop in a list of acidic pesticides which can be analyzed by a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) single residue method. 2,4-DP was separated by both Obelisc R and Primesep 100. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. Methods are LC/MS compatible and able to be used with many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dichlorprop (2,4-DP) |
HPLC Column Used
Primesep 100, 2.1×150 mm, 3 µm, 100A