Dicamba Analysis on Primesep and Obelisc Mixed-Mode HPLC Columns
https://sielc.com/Application-Dicamba-Analysis-on-Primesep-and-Obelisc-mixed-mode-HPLC-ColumnsChromatogram
Description
Dicamba is a widely used herbicide. Dicamba mimics auxin, a plant growth hormone, increasing growth beyond the resource availability of the plant. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for dicamba as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain dicamba and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing dicamba, other auxin-like phenoxy acids, and dozens of other pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4.5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dicamba |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A