Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns
https://sielc.com/Application-Controlling-Retention-of-2-4-DB-on-Mixed-Mode-HPLC-ColumnsChromatogram
Description
4-(2,4-dichlorophenoxy)butyric acid or 2,4-DB is a herbicides used in alfalfa, peanuts, and soybean production. 2,4-DB selectively controls broadleaf weeds inhibiting growth at the root and stem tips. It is considered slightly toxic, as is its metabolite 2,4-D. The EURL (European Union Reference Laboratory) included 2,4-DB in an analysis of acidic pesticides using QuEChERS methodology. Primesep 100 and Obelisc R were used to retain and separate 2,4-DB from impurities. Method is LC/MS compatible and developed to be used for many pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
|---|---|
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | 2,4-DB |
HPLC Column Used
Primesep 100, 2.1×150 mm, 5 µm, 100A