Complex of Cations on Primesep C is Effected by Organic Modifier
https://sielc.com/Application-Complex-of-Cations-on-Primesep-C-Is-Effected-By-Organic-ModifierChromatogram
Description
Primesep C separates a mixture of inorganic cations, potassium, sodium, and lithium, by a combination of cation exchange and complex formation. The peak order is reversed on the Primesep C column compared to the order normally obtained on columns that rely on cation exchange alone. In addition, the elution order can be rearranged by simply changing the organic modifier in the mobile phase from acetonitrile to methanol. The mobile phase mixture of water, acetonitrile (MeCN, ACN) or methanol (MeOH) and ammonium acetate is compatible with UV and evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeOH, MeCN |
|---|---|
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Lithium, Chloride |
HPLC Column Used
Primesep C, 4.6×50 mm, 5 µm, 100A