Chlorpyrifos-methyl Separation Using Obelisc and Primesep Columns
https://sielc.com/Application-Chlorpyrifos-methyl-Separation-Using-Obelisc-and-Primesep-ColumnsChromatogram
Description
Chlorpyrifos and Chlorpyrifos-methyl are amongst the most widely used pesticides. Chlorpyrifos is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EURL-CF9 and Obelisc R and Primesep B2 were used to separate chlorpyrifos-methyl and its impurities using a single multiresidue method, which can be used for many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing organophosphate pesticides.
Method Parameters
| Mobile Phase | Gradient MeCN – 10-70%, 15 min, 7 min hold |
|---|---|
| Buffer | Gradient AmAc pH 4,5- 20-50 mM, 15 min, 7 min hold |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Chlorpyrifos-methyl |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A