Bufferless Ion Separation (BLIS™) Chromatography of Amino Acids (2)
https://sielc.com/Application-Bufferless-Ion-Separation-BLIS-Chromatography-of-Amino-Acids-2Chromatogram
Description
Adding on to the previous HPLC separation of amino acids using Bufferless Ion Separation (BLIS) Chromatography; here we have additional amino acids separated on Primesep 200 column using only water and acetonitrile (MeCN, ACN) in the mobile phase. Primesep 200 is a reverse-phase (RP) column with weak acidic ion-pairing groups embedded. With no buffer present in the mobile phase, detection can be achieved with UV, mass spectrometry (MS), evaporative light scattering detection (ELSD).
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 195 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Aspartic acid, Alanine, Valine, Methionine, Leucine |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A