Separation of Toluidine and Aminobiphenyl Isomers
https://sielc.com/Application%20Separation%20of%20Toluidine%20and%20Aminobiphenyl%20IsomersChromatogram
Description
Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm Ph 3.5 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Hydrophobic, Ionizable |
| Analyzing Compounds | o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl |
HPLC Column Used
Primesep 200, 4.6×150 mm, 5 µm, 100A