HPLC Analysis of Promethazine
https://sielc.com/Application%20HPLC%20Analysis%20of%20PromethazineChromatogram
Description
Promethazine is a drug used as an antihistamine, a sedative, and used to reduce motion sickness, and prescribed for insomnia. The reverse-phase column Legacy L1 was used to retain promethazine. comparisons between Legacy L1 and Phenomenex columns are available upon request.
Method Parameters
| Mobile Phase | MeCN – 35% |
|---|---|
| Buffer | NaH2PO4 pH 2.5 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 254 nm |
| Class of Compounds | Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Promethazine |
HPLC Column Used
Legacy L1, 4.6×250 mm, 5 µm, 100A