HPLC Analysis of Kynurenine
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Description
Kynurenine is an important biological compound which carries out a number of biological functions including dilating blood vessles and regulating immune response. Kynurenine was retained on Legacy L1 which containes embedded C18 groups. Comparisons between this chromatagram and from Phenomenex columns are available upon request.
Method Parameters
| Mobile Phase | Gradient MeCN – 0-80%, 20 min |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 225 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Kynurenine |
HPLC Column Used
Legacy L1, 4.6×250 mm, 5 µm, 100A