HPLC Ananlysis of Hydrocortisone

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Chromatogram

Description

Hydrocortisone is the drug form of the steroid hormone cortisol. Cortisol is an important biological molecule which affects blood sugar, metabolism and the immune system. It was retained on the reverse-phase column Legacy L1. Comparisons of Legacy L1 and Phenomenex columns are available by request.

Method Parameters

Mobile PhaseMeCN – 40%
BufferNaH2PO4 pH 2.5 – 10 mM
Flow Rate1.0 ml/min
DetectionUV, 210 nm
Class of CompoundsHormone,   Hydrophobic, Ionizable
Analyzing CompoundsHydrocortisone

HPLC Column Used

Legacy L1, 4.6×250 mm, 5 µm, 100A
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