HPLC Analysis of Dichloran
https://sielc.com/Application%20HPLC%20Analysis%20of%20DichloranChromatogram
Description
Dichloran (DCNA) is a fungicide with low toxicity in humans, but is highly toxic to freshwater fish. Obelisc R was used to retain DCNA and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing DCNA.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 350 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dichloran |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A