HPLC Analysis of Chloramphenicol
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Description
Chloramphenicol is a broad-spectrum antibiotic useful in treating conjunctivitis and many other infections. It is notable as an antibiotic for developing nations due to its low cost. It was analyzed on a Legacy L1 column which uses reverse-mode to retain hydrophobic compounds. Comparisons between Legacy L1 and Phenomenex columns are available upon request.
Method Parameters
| Mobile Phase | MeOH – 45% |
|---|---|
| Buffer | Acetic Acid – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm |
| Class of Compounds | Drug, Antibiotics, Hydrophobic, Ionizable |
| Analyzing Compounds | Chloramphenicol |
HPLC Column Used
Legacy L1, 4.6×250 mm, 5 µm, 100A