HPLC Analysis of Azinphos-ethyl
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Description
Azinphos-ethyl is an organophosphate insecticide similar to Azinphos-methyl which was developed during World War II as a nerve agent. Due to risk concerns for farm workers and aquatic ecosystems AZM use was phased-out by the EPA in 2012. AZM is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Azinphos-ethyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing azinphos-ethyl and other organophosphate insecticides.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Azinphos-ethyl |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A