HPLC Analysis of Alachlor
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Description
Alachlor is a chloroacetanilide family herbicide which inhibits parts of the gibberellin pathway. The EPA classifies alachlor as slightly toxic and set the maximum contaminant level at 2 ppb. The EU banned alachlor in 2006. Obelisc R was used to retain alachlor and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Alachlor |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A