Analysis of Nicotinic Acid and Nicotinamide (Vitamin B3) on Newcrom AH Column
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Description
Nicotinamide is the amide form of nicotinic acid. Nicotinic acid is in the vitamin B group and is converted to nicotinamide in vivo. Newcrom AH was used to separate nicotinamide and nicotinic acid. Newcrom AH separates acids using ion-exclusion, retains basic compounds by cation-exchange, and neutral compounds by reverse-phase mechanism.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Nicotinic Acid, Nicotinamide, Vitamin B3 |
HPLC Column Used
Newcrom AH, 4.6×150 mm, 5 µm, 100A