HPLC Analysis of Cefepime

https://sielc.com/Applicatio-HPLC-Analaysis-of-Cefepime
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Chromatogram

Description

Cefepime is a fourth generation cephalosporin antibiotic usually reserved to treat severe nosocomial pneumonia and febrile neutropenia as well as multiple drug-resistant microorganisms such as Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Legacy L1 was used to retain Cefepime by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

Method Parameters

Mobile PhaseMeOH – 10%
BufferNaH2PO4 pH 5.0- 5 mM
Flow Rate1.0 ml/min
DetectionUV, 250 nm
Class of CompoundsDrug,  Hydrophilic, Ionizable
Analyzing CompoundsCefepime

HPLC Column Used

Legacy L1, 4.6×250 mm, 5 µm, 100A
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