Analysis of Lactic Acid on Primesep D Column
https://sielc.com/Applicatino-Analysis-of-Lactic-Acid-on-Primesep-D-ColumnChromatogram
Description
Lactic acid (milk acid) is a hydrophilic acid which plays important role in many bioprocesses. Lactic acid has a tendency to self-condense to form dimers, trimers and high molecular weight oligomers. Lactic acid and it’s oligomers are retained by a combination of weak reversed-phase and weak anion-exchange mechanisms on Primesep D mixed-mode HPLC column. Retention time is controlled by amount of acetonitrile, buffer concentration and buffer pH. Method is compatible with LC/MS and other detection techniques and can be used for analysis of lactic acid in various matrices.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | AmAc pH 4.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Lactic Acid |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A