Analysis of Antibiotic Tylosin and Related Impurities in Mixed-Mode Chromatography
https://sielc.com/Analysis_of_Antibiotic_Tylosin_and_Related_Impurities_in_Mixed-Mode_ChromatographyChromatogram
Description
Tylosin is a macrolide antibiotic with a broad spectrum of activity against gram-negative and gram-positive organisms. It is highly polar and basic molecule with limited retention in reversed-phase chromatography. Tylosin and related impurities were analysed on Obelisc R trimodal column. Method is compatible with LC/MS and prep chromatography and can be used for HPLC analysis of tylosin in various biofluids.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-30%, 10 min, 5 min hold |
|---|---|
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 5 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Tylosin |
HPLC Column Used
Obelisc R, 2.1×150 mm, 5 µm, 100A