Analysis of Antibiotic Marbofloxacin and Related Impurities in Mixed-Mode Chromatography
https://sielc.com/Analysis_of_Antibiotic_Marbofloxacin_and_Related_Impurities_in_Mixed-Mode_ChromatographyChromatogram
Description
Marbofloxacin is carboxylic acid derivative related to fluoroquinolone antibiotics. Method for analysis of marbofloxacin and related impurities was developed on Obleisc R and Primesep D mixed-mode column. Obelisc R is tri-modal reversed-phase, cation- and anion-exchange column. Primesep D is mixed-mode reversed-phase anion-exchange column.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Marbofloxacin |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A