Analysis of Antibiotic Josamycin and Related Impurities on Primesep D Column
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Description
Josamycin is a macrolide antibiotic effective against gram-positive bacteria. It is highly polar and does not retain well on reversed-phase columns. The Mixed-mode column Obelisc R was used to retain josamycin and its related impurities. Primesep D was also used to analyze josamycin impurities because Primesep D does not retain high-molecular weight plasma components. This method is compatible with LC/MS, prep chromatography and can be used for analysis of josamycin in biofluids.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50%, 10 min, 3 min hold |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Josamycin |
HPLC Column Used
Primesep D, 4.6×150 mm, 5 µm, 100A