Analysis of Antibiotic Dapsone and Related Impurities in Mixed-Mode Chromatography
https://sielc.com/Analysis_of_Antibiotic_Dapsone_and_Related_Impurities_in_Mixed-Mode_ChromatographyChromatogram
Description
Dapsone is an antibiotic used to treat leprosy and acne. Obelisc R was used to analyze dapsone and its impurities. Obelisc R is a tri-modal reversed-phase, cation- and anion-exchange column. Method is compatible with LC/MS and prep chromatography.
Method Parameters
| Mobile Phase | Gradient MeCN – 0-25%, 6 min, 25-70% 14 min |
|---|---|
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dapsone |
HPLC Column Used
Obelisc R, 4.6×150 mm, 5 µm, 100A