Analysis of Folic Acid and Related Impurities
https://sielc.com/Application-Analysis-of-Folic-Acid-and-Related-ImpuritiesChromatogram
Description
Folic acid is a water-soluble vitamin. It belongs to Group B of vitamins. Folate is composed of the aromatic pteridine ring linked to para-aminobenzoic acid and one or more glutamate residues. Folic acid (Vitamin B9) and related impurities were analyzed on the Primesep 100 mixed-mode HPLC column. This column can be used for analysis of this and other water-soluble vitamins. Several impurities and folic acid are well separated. The method is robust and can be used for analysis of folic acid in supplements and other compositions.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | H3PO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Folic Acid |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A